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. Author manuscript; available in PMC: 2019 Jun 26.
Published in final edited form as: J Allergy Clin Immunol. 2017 Sep 7;141(4):1395–1410. doi: 10.1016/j.jaci.2017.09.001

FIG 5.

FIG 5.

Depletion of B cells before challenge reduces the allergic response. A, Schematic representation of the experimental set-up. BALB/c mice were sensitized with HDM on day 0 and injected with α-CD20 or isotype-matched antibody on day 9 (α-CD20 and Ig Ctrl). Mice were challenged on days 11, 12, and 13 with PBS or HDM and analyzed on day 14. B, Absolute numbers of T cells (CD45+CD3+CD4+CD44+), eosinophils (CD45+CD11clo/−Siglec-F+CCR3+CD11bintGr-1int) and neutrophils (CD45+CD11bhiGr-1hiCD11c−Siglec-F−) in lungs were quantified by using flow cytometry. C, Lung CD4+CD44+ T cells were restimulated with HDM in cocultures with lung DCs for 4 days and cytokine production was measured by means of ELISA. IL-21 levels were less than the limit of detection. D, BAL fluid was collected on day 14, and cytokine production was measured by means of ELISA. IL-21 levels were less than the limit of detection. Data are pooled from 4 independent experiments. Graphs show the mean ± SEM. *P < .05, **P < .01, ***P < .001, and ****p < .0001.