Figure 3.

Enhanced systemic distribution of C. rodentium in mice pre-treated with amitriptyline. C57BL/6 mice were treated and infected as described in Figure 1. (A,B) Mice were orally administrated with FITC-labeled dextran beads. Serum was collected 4 h later, and fluorescence intensity was determined (n = 5). Serum FITC concentration in infected mice was normalized to serum FITC concentration of the respective control. (C,D) 5 and 10 dp C. rodentium infection spleen and liver were isolated and CFUs of C. rodentium were assessed (n = 13–27). (E–G) Concentration of the cytokines IL12p70, IFNγ, and TNFα in serum of WT and WT/Ami mice 10 dp C. rodentium infection were measured using Luminex technologies (n = 18–37). All data are presented as mean ± SEM. Statistics were performed using the Mann–Whitney test or Student's t-test (^*p < 0.05; ^**p < 0.01; ^***p < 0.001).