Figure 2.

Isolation of CD1b‐BbGL‐II binding T cells. (A) PBMC from blood donor BC24 (Table 1) with erythema migrans were sorted based on CD1b‐BbGL‐II tetramer binding. After three rounds of sorting and expansion, the 96% of CD4^‒ T cells that stain with CD1b‐BbGL‐II tetramers were further tested for staining with CD1b‐endo tetramers carrying endogenous (endo) lipids and an antibody against CD8α. Positive CD1b‐BbGL‐II tetramer, negative CD1b‐endo, and negative CD4 staining have been observed in five independent experiments. CD8 staining was performed once.