Figure 2.
Expression and phosphorylation of STAT proteins during moDC maturation. (a) Expression and phosphorylation of STAT1, STAT3, STAT5, and STAT6 on day 6, before addition of maturation stimuli, and day 7 after maturation with TLR ligands or cytokines, with tubulin used as loading control. One representative band of 4 donors is shown. (b) Quantification of STAT expression and phosphorylation, normalized to tubulin, shown as relative expression to day 6 moDCs (n = 4). Data are depicted as mean + SEM. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.