Table 4.
Evolution of the resistance acquisition of A. baumannii to colistin, considering the number of replicates alive after each day of exposure.
| Alive replicates after n days of exposure (%) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 | D10 | D11 | |
| Colistin | 100 | 100 | 100 | 97.5 | 97.5 | 97.5 | 97.5 | 97.5 | 65 | 65 | 35 |
| Colistin | |||||||||||
| + RNase 3/1 | 100 | 100 | 100 | 80 | 80 | 80 | 75 | 75 | 35 | 35 | 15 |
| Negative control | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Colistin dose | 0.1 μM | 0.25 μM | 0.3 μM | 0.35 μM | 0.7 μM | 2 μM | 20 μM | ||||
The initial number of replicate lineages was 40. Colistin dose was gradually increased according to bacterial growth, as detailed in the methodology. The colistin dose used in each exposure is indicated in the bottom row. RNase 3/1, when used, was kept constant at 0.1 μM.