Table 1.
Antibodies used for Immunofluorescent stainings.
| Antigen | Source | Cat.no | Clone | Isotype | Label | Species | Dilution |
|---|---|---|---|---|---|---|---|
| Antibodies used for immunofluorescent staining of in vivo material | |||||||
| GFP | AB | ab13970 | IgG | Chicken | 1/1,000 | ||
| Collagen I | AB | ab138492 | – | IgG | – | Rabbit | 1/200 |
| Collagen I | SA | C-2456 | COL-1 | IgG1 | – | Mouse | 1/100 |
| Antibodies used for immunofluorescent staining of in vitro material | |||||||
| WT1 | CA | CA1026 | – | IgG | – | Rabbit | 1/50 |
| αSMA | SA | a2547 | 1A4 | IgG2a | – | Mouse | 1/500 |
| Vinculin | SA | V9264 | hVIN-1 | IgG1 | – | Mouse | 1/400 |
| pFAK (Y397) | BD | bd-611723 | 14/FAKY397 | IgG1 | – | Mouse | 1/1,000 |
| Phalloidin | SA | 49409 | – | – | Atto 488 | Rabbit | 1/200 |
| Collagen I | SA | C2456 | COL-1 | IgG1 | – | Mouse | 1/100 |
| Collagen III | SA | ab7778 | – | IgG | – | Rabbit | 1/200 |
| Elastin | AB | ab23747 | – | IgG | – | Rabbit | 1/100 |
| Fibronectin | SA | F3648 | – | IgG | – | Rabbit | 1/200 |
| β1-Integrin | SC | sc-53711 | TS2/16 | IgG1 | – | Mouse | 1/50 |
| CD44 | AB | ab24504 | IgG | – | Rabbit | 1/100 | |
| Mouse IgG1 | IG | A21121 | IgG1 | Alexa Fluor 488 | Goat | 1/300 | |
| Rabbit IgG | IG | A31572 | IgG (H+L) | Alexa Fluor 555 | Donkey | 1/300 | |
| Rabbit IgG | MP | A11008 | IgG (H+L) | Alexa Fluor 555 | Goat | 1/200 | |
| Mouse IgG1 | MP | A21127 | IgG1 | Alexa Fluor 555 | Donkey | 1/300 | |
| Mouse IgG1 | MP | A21240 | IgG1 | Alexa Fluor 647 | Donkey | 1/250 | |
AB, Abcam; SA, Sigma Aldrich; CA, Calbiochem, BD, BD Biosciences; SC, Santa Cruz; IG, Invitrogen; MP, Millipore.