Table 3.

Expression cycle threshold Ct) for matrix proteins and modulators and differentiation markers of hEPDCs in different experimental conditions.

Gene	Experimental conditions
	Unstrained	Unstrained + TGFβ	Strained	Strained + iALK5	Strained + TGFβ
Collagen I	20.06 ± 1.02	19.35 ± 1.32	19.28 ± 0.77	21.47 ± 0.68	20.25 ± 1.16
Collagen III	22.91 ± 1.15	23.14 ± 1.20	22.77 ± 0.78	23.42 ± 1.51	24.25 ± 1.25
Fibronectin	22.00 ± 0.68	21.85 ± 0.93	21.90 ± 0.68	23.08 ± 0.90	22.98 ± 1.41
Elastin	36.97 ± 2.98	37.84 ± 2.49	38.66 ± 2.25	38.48 ± 2.27	35.90 ± 1.35
MMP-1	22.74 ± 1.87	23.96 ± 2.00	23.82 ± 1.45	23.39 ± 1.71	22.43 ± 1.33
MMP-2	23.00 ± 0.93	22.80 ± 1.95	22.89 ± 1.83	23.93 ± 1.16	23.69 ± 1.87
MMP-9	34.20 ± 3.74	35.41 ± 1.71	35.42 ± 1.60	35.22 ± 1.78	34.53 ± 1.11
TIMP-1	21.65 ± 0.94	21.87 ± 0.83	21.67 ± 0.74	21.84 ± 0.92	–
TIMP-2	23.31 ± 0.38	23.60 ± 0.84	23.28 ± 0.55	23.99 ± 0.75	23.66 ± 0.35
TIMP-4	33.03 ± 0.69	31.94 ± 0.56	32.72 ± 0.68	33.71 ± 0.72	–
Pai-1	22.73 ± 1.30	22.08 ± 1.30	21.76 ± 1.01	23.75 ± 1.30	22.92 ± 1.81
WT1-isoA	29.28 ± 2.35	30.38 ± 2.58	30.43 ± 2.46	29.63 ± 3.43	32.07 ± 2.42
αSMA	24.93 ± 1.16	24.24 ± 1.70	24.46 ± 1.25	26.71 ± 0.59	25.58 ± 1.56

The cycle threshold Ct) for each gene varied over the different experimental conditions and between patients. Not applicable Ct values were set at a value of 40. The table represents mean Ct values + SD. Genes that are highly expressed gave a Ct value <20, moderate expression was shown by Ct values of 20–26. Low and hardly expressed genes gave a high Ct value of 26–34 and 34–40, respectively.