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. 2019 Jun 19;10:803. doi: 10.3389/fpls.2019.00803

FIGURE 2.

FIGURE 2

Diagrams showing different replicon-based expression systems. (A) Deconstructed monopartite RNA virus, TMV-based system, where the viral CP was substituted with the GOI under the control of a subgenomic promoter (Lindbo, 2007). (B) Deconstructed monopartite RNA virus, PVX-based system, where the viral CP and MP were substituted with the GOI under the control of a subgenomic promoter (Komarova et al., 2006). (C) Deconstructed bipartite RNA plant virus, CPMV-based system, where the RNA-1 component supplies the viral RdRp, virus genome-linked protein, helicase, and protease, while the modified RNA-2 contains the GOI, replacing those of the viral MP and CP (Cañizares et al., 2006). (A) Described replicon design is capable of replication, cell-to-cell movement, transcription, and replication, while designs (B,C) are capable of all, but deficient in cell-to-cell movement. LB, left border; RB, right border of the T-DNA region.