FIGURE 5:

Chromatin condensation factors can be efficiently loaded on mitotic chromatin in the presence of the MCT. Synchronized MCT/H2B* cells were released into mitosis as outlined in Figure 1B. Cells were treated with DMSO or rapamycin 8 h after release, fixed 2 h after the treatment, and immunostained using anti-SMC2 (A), anti-topoisomeraseII (B) and anti–Ki-67 (C) antibodies and analyzed by confocal microscopy. Chromatin configuration was used to assign cells to the indicated cell cycle stages. Dashed lines represent spindle axes. Bars, 10 μm.