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. 2019 May 10;160(7):1757–1770. doi: 10.1210/en.2019-00238

Figure 1.

Figure 1.

Changes in Crh gene expression within the PVN following ADX of male and female mice. PVN CRH mRNA levels were evaluated using (a–d) in situ hybridization or (e) ddPCR in ADX’d male and female mice vs sham-operated controls. (a and b) Representative autoradiograms of CRH mRNA hybridization in sham, 2-day ADX, or 4-day ADX (a) male or (b) female mice. (c and d) AdUs calculated from autoradiograms for (c) males and (d) females. Bars represent mean ± SEM AdUs in the PVN of n = 5 to 9 individuals. **P < 0.01; ***P < 0.001; ****P < 0.0001. (e) Mean absolute levels ± SEM of PVN CRH mRNA in n = 5 to 8 male mice, 2 days after ADX or sham surgery. Absolute CRH mRNA levels were measured by ddPCR and normalized to the amount of input cDNA. *P < 0.05 for ADX’d males compared with controls. 3V, third ventricle; OT, optic tract.