Table 1.

Sequences of primers used in the real‐time polymerase chain reaction

Genes	Forward primer	Reverse primer
Runx2 (234 bp)	5′‐GTCCCAACTTCCTGTGCTCC‐3′	5′‐TCTTGCCTCGTCCGCTCC‐3′
ColIIa1 (115 bp)	5′‐ACTGGTAAGTGGGGCAAGAC‐3′	5′‐CCACACCAAATTCCTGTTCA‐3′
MMP13 (173 bp)	5′‐CTTCTTCTTGTTGAGCTGGACTC‐3′	5′‐CTGTGGAGGTCACTGTAGACT‐3′
Adamts5 (110 bp)	5′‐GGAGCGAGGCCATTTACAAC‐3′	5′‐CGTAGACAAGGTAGCCCACTTT‐3′
ColXa1 (138 bp)	5′‐CTTTGTGTGCCTTTCAATCG‐3′	5′‐GTGAGGTACAGCCTACCAGTTTT‐3′
Aggrecan (150 bp)	5′‐CCTGCTACTTCATCGACCCC‐3′	5′‐AGATGCTGTTGACTCGAACCT‐3′
β‐actin (241 bp)	5′‐CACGATGGAGGGGCCGGACTCATC‐3′	5′‐TAAAGACCTCTATGCCAACACAGT‐3′