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. 2019 Jun 26;14(6):e0217709. doi: 10.1371/journal.pone.0217709

Fig 4. A monolayer of cells lines the luminal surface of complex-shaped BVMs.

Fig 4

After pressure-sodding HUVECs onto the luminal surface of L- and U-shaped scaffolds and cultivating the angulated BVMs for 1 day, a monolayer of cells was visible. (A–B) On the inner curve of the angulated vessels, cells were visible in cross-sectional H&E images (A) and in en face SEM images (B) (n = 3). (C–D) On the outer curve of the angulated BVMs, cells were also visible in cross-sectional H&E images (C) and in en face SEM images (D) (n = 3). The SEM images show the surface of the ePTFE scaffolds: long vertical lines are the “nodes” of the scaffold, and short horizontal lines are the “internodal fibers.” The SEM images also show the cells sprawling across the scaffold nodes and fibers. The amount of cells appeared similar on the inner and outer halves of the angulated BVMs.