Fig 7. Bioreactor prototypes for bifurcated BVMs.

(A–B) Bifurcated scaffolds were created by suturing together two straight scaffolds using 6.0 silk suture. (C–D) The bifurcated scaffold was secured into a T-shape in the multifunctional bioreactor. As with the angulated BVMs, luer caps were used to close unused inlets and outlets in the multifunctional bioreactor. (E–F) A separate bioreactor was constructed to house Y-shaped BVMs. Custom Y-shaped bioreactor construction was simple and straightforward using the methods presented in this paper. To create a Y-shaped scaffold, the T-shaped scaffold was simply bent into a Y-shape.