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. 2019 Jun 26;14(6):e0218261. doi: 10.1371/journal.pone.0218261

Fig 1. Newly established familial PD model flies express equivalent amounts of WT or mutant αSyn mRNA.

Fig 1

(A) Diagram showing αSyn and the locations of the PD-linked mutations used in this study. (B, C) Comparison of relative αSyn mRNA levels demonstrating equivalent expression levels between transgenic flies expressing either WT αSyn or each of the mutants. αSyn was expressed either in compound eyes using the GMR-GAL4 driver (B) or pan-neuronally using the nSyb-GAL4 driver (C). Total RNA was obtained from 1-day old male adult fly heads, and used for reverse transcription and quantitative PCR. Each value was normalized to the amount of a ribosomal protein gene Rpl32 mRNA. The expression level of WT αSyn was set to 1.0. Data are expressed as the mean ± s.e.m. Fly genotypes in (B): WT, GMR-GAL4/Y;;UAS-hWT αSyn/+; A30P, GMR-GAL4/Y;;UAS-hA30P αSyn/+; A53T, GMR-GAL4/Y;;UAS-hA53T αSyn/+; E46K, GMR-GAL4/Y;;UAS-hE46K αSyn/+; G51D, GMR-GAL4/Y;;UAS-hG51D αSyn/+; H50Q, GMR-GAL4/Y;;UAS-hH50Q αSyn/+, fly genotypes in (C): WT, +/Y;;UAS-hWT αSyn/nSyb-GAL4; A30P, +/Y;;UAS-hA30P αSyn/nSyb-GAL4; A53T, +/Y;;UAS-hA53T αSyn/nSyb-GAL4; E46K, +/Y;;UAS-hE46K αSyn/nSyb-GAL4; G51D, +/Y;;UAS-hG51D αSyn/nSyb-GAL4; H50Q, +/Y;;UAS-hH50Q αSyn/nSyb-GAL4.