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. 2019 Jun 26;14(6):e0218261. doi: 10.1371/journal.pone.0218261

Fig 5. Delayed E46K αSyn degradation compared with WT αSyn degradation.

Fig 5

(A) Time course of the conditional expression of αSyn in flies. αSyn expression was transiently induced by the administration of RU486 during the larval stage, and subsequently stopped by withdrawing RU486 during the pupal and adult stages. Adult male flies were collected on day 1, 4, and 7 after eclosion. (B) Immunoblotting analysis of adult fly head lysates using an anti-αSyn antibody. The transient expression of αSyn and time-dependent decrease in αSyn protein levels was observed. (C) The time-dependent protein degradation rate of E46K αSyn was compared with that of WT αSyn. A decrease in undegraded WT αSyn protein levels was observed on day 4, whereas E46K protein levels remained almost unchanged. *P < 0.05 (Student t-test). Fly genotypes: WT, GMR-GeneSwitch/Y;;UAS-hWT αSyn/+; E46K, GMR-GeneSwitch/Y;;UAS-hE46K αSyn/+.