Fig 7. CLR collaboration is responsible for the protective effect of inflammatory monocytes during infection with C. albicans.

(A) Quantification of fungal burden in the kidneys (left) or brains (right) of WT and Ccr2 KO mice 2 days after intravenous infection with 1x10⁴ CFU C. albicans. Graphs display cumulative data from 2 independent experiments. Each symbol represents one mouse. (Student’s t test on transformed data). (B) Protocol of WT or Min-D2-D1 TKO1 monocyte transfer and C. albicans infection in Ccr2 KO mice. (C) 3-4x10⁶ purified Ly6C^hi monocytes from WT or Min-D2-D1 TKO1 mice were adoptively transferred to Ccr2 KO mice followed by intravenous injection with 1.5x10⁴ CFU C. albicans. Organs were harvested after 72 h and fungal burden was determined. Graphs display cumulative data from 3 independent experiments. Each symbol represents one mouse. (Student’s paired t test). (D-F) 2.5x10⁶ purified monocytes from WT or Min-D2-D1 TKO1 mice were adoptively transferred to Ccr2 KO mice followed by intravenous injection with 1.5x10⁴ CFU C. albicans. (D-E) Kidneys were harvested after 24 h and myeloid cell recruitment to the kidneys was determined by flow cytometry. (F) Fungal burden and chemokine/cytokine production in the kidneys was determined. Graphs display cumulative data from 3 independent experiments. Each symbol represents one mouse. (Student’s t test).