Fig 1. The NTF2-like and RGG domains of G3BP are necessary for efficient Old World alphavirus replication.

A. Schematic of GFP-G3BP1 constructs used for reconstitution of G3BP1/2 double KO cell lines. B. Indicated cell lines were lysed and analysed by SDS-PAGE and immunoblotting for GFP and β-tubulin. C. and D. Indicated cells lines were infected with WT SFV (C) or WT CHIKV (D) at a multiplicity of infection (MOI) of 0.1. At 4, 8, 12, 24 and 36 hpi, supernatants were collected, and viral titres were quantified by plaque assay on BHK cells. Data are means of three independent experiments. Error bars indicate standard error of mean (SEM). For statistical analysis samples within each timepoint were compared to the respective titre obtained in U2OS ΔΔG3BP1/2 + GFP-G3BP1 WT cells. ns P>0.05, *P≤0.05, **P≤0.01, ***P≤0.001.