Fig 2. The NTF2-like and RGG domains of G3BP are differently required for efficient formation of SFV and CHIKV dsRNA-positive replication complexes.

A. Indicated cell lines were infected with WT SFV at MOI 10. At 8 hpi cells were fixed and stained for nsP3 (red) and dsRNA (blue). Representative images from 3 independent experiments are shown. B. DsRNA signal intensities from images shown in A. were quantified using CellProfiler software. Bars represent mean + SEM for n = 50 cells per cell line. Individual dsRNA intensities for each analysed cell are shown as grey dots. ns P>0.05, *P≤0.05, **P≤0.01, ***P≤0.001. C. Indicated cell lines were infected with CHIKV at MOI 1. At 8 hpi cells were fixed and stained for nsP3 (red) and dsRNA (blue). Representative images from 3 independent experiments are shown.