Fig 6. Conserved transcript expression of core CAM genes and recruitment of PPC during CAM evolution.

(A-C) Transcript expression of PPC, PPCK and MDH genes in S. album, A. comosus and K. fedtschenkoi are plotted. (D) Maximum likelihood phylogenetic tree of PPC orthologs in 10 species. The bootstrap support values are indicated on each node. The PPC copies used by CAM and C4 plants are highlighted in blue and orange, respectively. Clock associated cis-elements located in the 2kb upstream promoter of PPC are denoted in blue (morning element, ME), yellow (TCP transcription factor), and green (MYB transcription factor).