Magnetic imaging of individual natural hemozoin nanocrystals. (a) SEM
images of hemozoin nanocrystals n1–n5, labeled in Fig. 3. (b) Corresponding diamond-magnetic-microscopy
images (B0 = 186 mT) for each crystal. (c) Simulated
magnetic images (χ = 3.4 ×
10−4, B0 = 186 mT) obtained with
nanocrystal dimensions inferred from (a). Only the crystal at the center of each
SEM image is included in the model. (d) Line cuts of each nanocrystal (red,
measured; gray, simulated), from which the field-pattern amplitude
ΔB is inferred. A minimum feature width of ~
400-nm FWHM is observed for n5, close to the optical diffraction-limited
resolution of our microscope. (e)
ΔB(B0) = 0
(zero-coercivity assumption). (f) Histogram of ΔB (B0 =
350 mT) of the 78 crystals exhibiting linear paramagnetic
behavior (including n1–n4). The four crystals exhibiting
superparamagnetic behavior (including n5) are excluded from the analysis. (g)
Fitted slopes, dΔB/dB0, as a function
of crystal area, A, as determined from SEM images. The data are
fitted with an empirical saturation function,
dΔB/dB0 =
Smax/(1 +
Asat/A), with
Smax = 16.5 ± 1.4
μT/T and
Asat = 0.17 ± 0.03
μm2.