Table 2.
Evaluation of semi-selective LOMAC agar medium for the cultivation of Pantoea spp. from environmental soils
| Soil | LOMAC agar medium | Species identified by the biochemical test Api20E (No. of isolates tested) | % Positive predictive value | % Negative predictive value | % Overall agreement | |
|---|---|---|---|---|---|---|
|
| ||||||
| Total no. of colonies | No. of yellow colonies | |||||
| A | 55 | 5 |
Yellow colonies: Pantoea sp3 (5) Non-yellow colonies: Aeromonas hydrophila/caviae/sobria (1), Bordetella/Alcaligenes/Moraxella spp. (2), Enterobacter amnigenus (3), Ochrobacterium anthropi (1), Photobacterium damselae (1), Pseudomonas aeruginosa (6), Pseudomonas fluorescens/putida (31), Pseudomonas luteora (3), Pseudomonas oryzihabitans (1), Stenotrophomonas maltophilia (1) |
100% (5/5) | 100% (50/50) | 100% (55/55) |
| B | 30 | 4 |
Yellow colonies: Pantoea sp3 (1), Citrobacter youngae (2), Leclercia adecarboxylate (1), Non-yellow colonies: Acinetobacter baumannii/calcoaceticus (5), Enterobacter cloacae (10), Hafnia alvei (1), Klebsiella pneumoniae (1), Pseudomonas aeruginosa (1), Pseudomonas fluorescens/putida (8) |
25% (1/4) | 100% (26/26) | 90% (27/30) |
| C | 14 | 1 |
Yellow colonies: Pantoea sp3 (1) Non-yellow colonies: Pseudomonas fluorescens/putida (13) |
100% (1/1) | 100% (13/13) | 100% (14/14) |
| 99 | 10 | 17 species | 70% (7/10) | 100% (89/89) | 97% (96/99) | |