Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Danio rerio) | pappaap170 | Wolman et al., 2015 | RRID:ZFIN_ZDB-GENO-190322-4 | single nucleotide nonsense mutationC > T at position 964 in Exon 3 |
| Strain, strain background (Danio rerio) | Tg(myo6b:mitoGCaMP3) | Esterberg et al., 2014 | RRID:ZFIN_ZDB-GENO-141008-1 | |
| Strain, strain background (Danio rerio) | Tg(brn3c:GFP) | Xiao et al., 2005 | RRID:ZFIN_ZDB-ALT-050728-2 | |
| Strain, strain background (Danio rerio) | Tg(mnx1:GFP) | Rastegar et al., 2008 | RRID:ZFIN_ZDB-GENO-140605-2 | |
| Strain, strain background (Danio rerio) | Tg(hsp70:dnIGF1Ra-GFP) | Kamei et al., 2011 | RRID:ZFIN_ZDB-GENO-110614-4 | |
| Strain, strain background (Danio rerio) | Tg(hsp70:pappaa-GFP) | this paper | Materials and methods subsection maintenance of zebrafish | |
| Strain, strain background (Danio rerio) | TLF | Zebrafish International Resource Center (ZIRC) | RRID:ZFIN_ZDB-GENO-990623-2 | |
| Antibody | anti-myosinVI (rabbit polyclonal) | Proteus biosciences | RRID:AB_10013626 | 1:200 |
| Antibody | anti-SOX2 (rabbit polyclonal) | Abcam | RRID:AB_2341193 | 1:200 |
| Antibody | anti-GFP (rabbit polyclonal) | ThermoFisher Scientific | RRID:AB_221569 | 1:500 |
| Antibody | Alexa 488, secondary (rabbit polyclonal) | ThermoFisher Scientific | RRID:AB_2576217 | 1:500 |
| Peptide, recombinant protein | IGF-1 | Cell sciences | Catalog number: DU100 | |
| Other | FM1-43 | ThermoFisher Scientific |
Catalog number: T3136 PubChem CID:6508724 |
3 uM for 30 s |
| Other | CellROX deep red | ThermoFisher Scientific | Catalog number: C10422 | 10 uM for 60 min |
| Other | mitoSOX red | ThermoFisher Scientific | Catalog number: M36008 |
1 uM for 30 min |
| Other | mitotracker greenFM | ThermoFisher Scientific | Catalog number: M7514 PubChem CID:70691021 |
100 nM for 5 min |
| Other | TMRE | ThermoFisher Scientific | Catalog number: T669 PubChem CID:2762682 | 25 nM for 20 min |
| Other | 0.25% trypsin-EDTA | Sigma-Aldrich | Catalog number: T3924 | |
| Other | TRIzol | Invitrogen | Catalog number: 15596026 | |
| Other | Sso fast Eva Green Supermix | BioRad | Catalog number: 1725200 | |
| Chemical compound, drug | Neomycin | Sigma-Aldrich | Catalog number: N1142 PubChem CID:24897464 | |
| Chemical compound, drug | NBI-31772 | Fisher Scientific | Catalog number: 519210 | |
| Chemical compound, drug | antimycin-a | Sigma-Aldrich | Catalog number: A8674 PubChem CID: 24891355 |
|
| Chemical compound, drug | CsA | Abcam | Catalog number: ab120114 PubChem ID: 5284373 |
|
| Chemical compound, drug | mitoTEMPO | Sigma-Aldrich | Catalog number: SML0737 PubChem CID: 134828258 |
|
| Chemical compound, drug | NVP-AEW541 | Selleck | Catalog number: S1034 PubChem CID: 11476171 |
|
| Commercial assay or kit | SuperScript II Reverse Transcriptase | Invitrogen | Catalog number: 18064014 | |
| Software, algorithm | Fluoview (FV10-ASW 4.2) | Olympus | RRID:SCR_014215 | |
| Software, algorithm | ImageJ | PMID: 22743772 | RRID:SCR_003070 | |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 |