Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Biological sample (Xenopus laevis) | Xenopus laevis oocytes | African Reptile Park | RRID: NXR_0.0080 | mandyvorster@xsinet.co.za |
Commercial assay or kit | HiSpeed Plasmid Midi Kit | Qiagen | 12643 | |
Commercial assay or kit | QuickChange II Mutagenesis Kit | Agilent Technologies | 200524–5 | |
Commercial assay or kit | mMESSAGE mMACHINE T7 Transcription Kit | ThermoFisher | AM1344 | |
Chemical compound, drug | Collagenase type II | Gibco | 17107–0125 | |
Chemical compound, drug | Adenosine 5′-triphosphoribose magnesium (ATP) | Sigma-Aldrich | A9187 | |
Chemical compound, drug | Protein kinase A catalytic subunit, bovine | Sigma-Aldrich | P2645 | |
Chemical compound, drug | Vx-770 (solid) | Selleck Chemicals | S1144 | |
Chemical compound, drug | Vx-770 (10 mM in DMSO) |
Selleck Chemicals | S1144 | |
Software, algorithm | Pclamp9 | Molecular Devices | RRID: SCR_011323 |