Table 2. Model fit parameters for Schemes 3 and 4.
Average fit parameters (mean ± S.E.M.) obtained from global fits of Schemes 3 (left column) and 4 (right column) to three pairs of macroscopic WT CFTR current activation/deactivation time courses recorded in response to addition/removal of 0.01x saturated and 1x saturated concentrations of Vx-770 to the cytosolic (bath) solution. * Parameters kin and kout were constrained through kin/kout = Doct/wat(pH=7.1) = 62580. ** Parameters Kd;mem, K0.5;mem, and K0.5;aq were calculated from the fit parameters as described in Materials and methods.
| Scheme | 3 | 4 |
|---|---|---|
| kin (s-1)* | 4034 ± 464 | 9794 ± 531 |
| kout (s-1)* | 0.06446 ± 0.00742 | 0.1565 ± 0.0085 |
| kon' (M-1s-1) | 870.3 ± 104.0 | 3703 ± 627 |
| koff (s-1) | 0.007451 ± 0.000871 | 0.01075 ± 0.00009 |
| kflip (s-1) | - | 0.04408 ± 0.00583 |
| Po;max/Po;bas | 3.935 ± 0.001 | 3.996 ± 0.007 |
| Kd;mem (µM)** | 8.751 ± 1.160 | 3.106 ± 0.605 |
| K0.5;mem (µM)** | 21.13 ± 2.80 | 7.498 ± 1.460 |
| K0.5;aq (nM)** | 0.6752 ± 0.0895 | 0.6690 ± 0.0955 |