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. 2019 Jun 9;11(11):3639–3649. doi: 10.18632/aging.102003

Figure 6.

Figure 6

miR-186-5p is involved in MORC2-mediated CCA cell growth and metastasis. (A) CCK-8 analysis of cell viability after transfection with miR-186-5p inhibitor and sh-MORC2 plasmid in the HuCCT1 cell line. (B–C) Transwell analysis of cell invasive potential after transfection with miR-186-5p inhibitor and sh-MORC2 plasmid in the HuCCT1 cell line. (D–E) Western blot analysis of the levels of p-AKT, AKT, Slug, E-cadherin, N-cadherin and Vimentin after transfection with miR-186-5p inhibitor and sh-MORC2 plasmid in the HuCCT1 cell line. All experiments were performed in triplicate, **P < 0.01, *P < 0.05.