Figure 2. Role of mTOR activity in white adipose tissue of Lmna^+/+ and Lmna^−/− mice treated with rapamycin.

(A-C) Western blots of mTORC1 activity in white adipose tissue (WAT) of Lmna^+/+ and Lmna^−/− mice treated with rapamycin. Relative p-S6 levels (normalized to S6) were quantified. (D-F) Western blots of mTORC2 activity in WAT of Lmna^+/+ and Lmna^−/− mice treated with rapamycin. Relative p-Akt levels (normalized to Akt) were quantified. Each value is mean ± SEM for the number of mice indicated in parentheses. P values were derived from unpaired two-tailed Student’s t-test. ns: no significance.