Figure 3. Rapamycin reverses the elevated lipolysis in white adipose tissue of Lmna^−/− mice.

(A) Simplified diagram of the lipolytic pathway in white adipose tissue (WAT). TG: triglyceride. DG: diglyceride. MG: monoacylglycerol. ATGL: adipose triglyceride lipase. HSL: hormone-sensitive lipase. FFA: free fatty acid. (B-D) Activity of lipolysis, indicated by ATGL, of Lmna^+/+ and Lmna^−/− mice treated with rapamycin. Relative ATGL levels (normalized to GAPDH) were quantified. (E-G) Activity of lipolysis, indicated by p-HSL, of Lmna^+/+ and Lmna^−/− mice treated with rapamycin. Relative p-HSL levels (normalized to HSL) were quantified. (H) FFA of Lmna^+/+ and Lmna^−/− mice treated with rapamycin. (I) Western blots of ATGL levels in preadipocytes derived from WAT of Lmna^−/− mice. Representative blots were shown from 5 Lmna^−/− mice. Rapa: rapamycin. Each value is mean ± SEM for the number of mice indicated in parentheses. P values were derived from unpaired two-tailed Student’s t-test.