Figure 4. MiR-615–5p regulates the expression of downstream AKT/eNOS signaling in ECs.
HUVECs transfected with (A) miR negative control (NSm) or miR-615–5p mimics (miR-615–5pm) or (B) miR inhibitor negative control (NSi) or miR-615–5p inhibitor (miR-615–5pi) were subjected to Western analysis using antibodies to p-AKT, p-eNOS, AKT, eNOS, p-p38, p38, p-ERK1/2, ERK1/2 and β-actin (n = 3 to 5 experiments). (C) NO release was measured by Griess assay. All data represent mean ± s.e.m. * P < 0.05 compared to controls.