Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 May 16;39(7):1458–1474. doi: 10.1161/ATVBAHA.119.312726

Figure 6. SiRNA-mediated knockdown of IGF2 and RASSF2 recapitulates miR-615–5p functional effects in ECs.

Figure 6.

(A) HUVECs were transfected with siRNA to RASSF2 (A-C), IGF2 (D-F), IGF2 and RASSF2 (G) or scrambled control (ctrl) siRNA. Protein expression was determined by Western analysis under baseline conditions (A, D) or in response to VEGF treatment (B,E) using antibodies to RASSF2, IGF2, p-Akt, Akt and GAPDH (n = 2 experiments). *P < 0.01. (C,F,G) migration of ECs were quantified by scratch assay. *P < 0.01. Results are representative of n = 3 replicates per group. All data represent means ± s.e.m.