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. Author manuscript; available in PMC: 2020 Jun 21.
Published in final edited form as: Neurosci Lett. 2019 Mar 30;704:67–72. doi: 10.1016/j.neulet.2019.03.052

Figure 1.

Figure 1.

Intraperitoneal ganciclovir ablates adult hippocampal neurogenesis.

A) Experiment schedule for quantification of NPC ablation.

B) Fluorescent immunohistochemistry for DCX, a marker of immature neurons that derive from GFAP-positive NPCs [15], in brains collected one day after the FST. Daily ganciclovir depletes DCX-positive NPC progeny in the dentate gyrus, indicating ablation of hippocampal neurogenesis. Scale bar: 100um.

C) Quantification of NPC ablation from mice treated with BrdU (after completing behavior testing). BrdU+ DCX+ double-positive cells in the SGZ are depleted in GFAP-TK mice compared to wild-type. Lithium treatment does not change the extent of NPC ablation. Bar graph and error bars represent mean ± SEM; n = 4 per group. Individual data points are color-coded by sex (red = male, blue = female). Significance: ** p < 0.005 by Welch’s two sample t-test. OF, Open Field; FST, Forced Swim Test; I.P., intraperitoneal; DCX, Doublecortin.