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. 2019 Jun 26;9:9307. doi: 10.1038/s41598-019-45793-w

Figure 8.

Figure 8

A competitive study between FN (2.5 μM) and p38 inhibitor (10 μM) upon myogenic enhancement in C2C12 cells. Cells treated with FN in the presence/absence of SB203580 (10 µM) for 48 h. Proteins were resolved by SDS-PAGE and incubated with specific antibodies targeted against pp38, myoD, myogenin, and α-tubulin for immunoblotting analysis. (a) Key myogenic proteins expression in the experimental cells. (b) Quantitative analysis of protein bands was performed using the ImageJ program. (c) Myotubes formation in the experimental cells. Bars represent mean ± SEM of three experimental replicates. Different letters a, b, c and d within a column indicate significant differences between treatment and non-treatment groups (p < 0.05).