Fig. 5.

Loss of H3 lysine 79 methylation 2/3 (H3K79me2/3) leads to a reduction in H3K79me2/3 Enhancer Element (KEE)–promoter interactions. a Capture-C (n = 3) at ARID1B in SEM and RS4;11 cells in control (black) and DOT1Li (orange) conditions. Differential track shows average difference in Capture-C signal following DOT1Li, from three biological replicates. Loss of interaction (pink), and gain of interaction (black). H3K79me2, H3 lysine 27 acetylation (H3K27ac), H3K4me1 chromatin immunoprecipitation-sequencing (ChIP-seq) at ARID1B. Red line represents location of Capture-C probe. b Capture-C (n = 3) and ChIP-seq at LMO4 in SEM and RS4;11 cells, as in a. c Overlay of control (black) and DOT1Li (orange) Capture-C signal (average of three biological replicates) from the ARID1B and LMO4 promoters in SEM and RS4;11 cells. Gray bars represent location of Capture-C probe, ±1 kb exclusion zone. Shaded area around Capture-C signal represents 1 s.d. See also Supplementary Fig. 5