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. 2019 Jun 26;10(7):504. doi: 10.1038/s41419-019-1687-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Co-immunoprecipitation of SPARC with GRP78 in (a) HCT116 and confirmed in (b) MIP/Zeo and MIP/SP cells. (c) Colocalization of (i) GRP78 and (ii) SPARC in HCT116 by confocal microscopy and immunofluorescent analysis. (iii) The endoplasmic reticulum was stained with ER tracker blue-white DPX dye. (iv) Images were overlapped, and (v) the GRP78:SPARC colocalization pixels were identified by Colocalization Finder plugin in ImageJ and highlighted with yellow circles. The average ER tracker staining intensity in the highlighted areas was calculated to determine the percentage of GRP78:SPARC colocalization in the ER. Scale bar = 5 μm