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. 2019 Jun 26;10(7):504. doi: 10.1038/s41419-019-1687-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — The effects of SPARC and GRP78 expression on cell viability under ER stress were determined by using MIP/SP and GRP78-overexpressing MIP/SP cells (MIP/SP/78) following exposure to 5-FU (5 μM) and TM (1 μg/ml) for 48 h. Cell viability and apoptosis were analyzed by (a) MTS and (b) TUNEL assays, respectively. Cell viability and IC₅₀ of CRC cell lines HT29, CCL227, and HCT116 following exposure to (c) 5-FU and (d) CPT-11 were determined by MTS assay. The mRNA expression levels of GRP78 (left), SPARC (middle), and the relative expression level (GRP78:SPARC) (right) in (e) CRC cell lines (HT29, CCL227, HCT116), (f) MIP101, and (g) RKO 5-FU- and CPT-11-resistant cell lines were determined by q-RT-PCR. (The data represent mean ± SEM, N = 3)