Table 2.

Primer sequence used for quantitative real-time PCR analyses

Target bacterial species	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)	Amplicon size (bp)
Total bacteriaa	ACTCCTACGGGAGGCAGCAGT	ATTACCGCGGCTGCTGGC	174
F. succinogenes b	GGTATGGGATGAGCTTGC	GCCTGCCCCTGAACTATC	446
R. albus b	CCCTAAAAGCAGTCTTAGTTCG	CCTCCTTGCGGTTAGAACA	175
R. flavefaciens b	TCTGGAAACGGATGGTA	CCTTTAAGACAGGAGTTTACAA	295

F. succinogenes, Fibrobacter succinogenes; R. albus, Ruminococcus albus; R. flavefaciens, Ruminococcus flavefaciens

^aSchwiertz et al. (2010)

^bKoike and Kobayashi (2001)