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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Mol Cell. 2008 Dec 5;32(5):718–726. doi: 10.1016/j.molcel.2008.10.025

Figure 4: RNAi knockdown of CDYL augments transformation of immortalized primary human squamous epithelial cells (NOK) by HPV16 oncoproteins E6 and E7.

Figure 4:

(a) Normal Oral Keratinocyte (NOK) transformation assay. hTERT-immortalized primary human squamous epithelial cells (NOK) were engineered to stably express HPV16 oncoproteins E6 and E7 using retroviral transduction (E6/E7-NOK). These cells were then stably transduced with two independent shRNA vectors targeting knockdown of CDYL. As a negative control, cells were stably transduced with shRNA vectors targeting firefly luciferase (shFF2). Cells were plated in semi-solid media to assay for anchorage-independent (A.I.) growth. (b) Immunoblotting confirmed shRNA knockdown of CDYL in E6/E7-NOK. (c) Transformation assay showing that shCDYL augments A.I. colony formation of E6/E7-NOK. Shown is the mean of triplicate samples +/− s.d.