Figure 1.

Gα_q Is Crucial for the Cold Sensitivity of TRPM8⁺ DRG Neurons

(A–D) Firing responses evoked by two consecutive cold ramps and WS-12 (1 μM) in wild-type (WT) (A and B) and Gα_q^−/− (C and D) DRG neurons in the absence (A and C) or presence (B and D) of PBMC (50 nM). Arrows indicate cold activation threshold.

(E) Histogram distribution of cold activation threshold of DRG neurons from experiments similar to those in (A)–(D). WT, n = 25; Gα_q^−/−, n = 24.

(F) Bar summary of threshold for cold activation of DRG neurons.

(G and H) Summary of total number of firing (G) and average peak amplitude of firing (H) responses evoked during cold application period from the same experiments as described above.

(I) Summary of total number of firing responses evoked during capsaicin application (0.5 μM, 10 s).

Error bars in all figures represent mean ± SEM. ^∗p < 0.05; ^∗∗∗p < 0.001; NS, not significant. See also Figure S1.