REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-HA.11	Covance	Cat# MMS-101R-1000; RRID:AB_291262
Mouse monoclonal anti-β-Tubulin	Sigma-Aldrich	Cat# T4026; RRID:AB_477577
Rabbit polyclonal anti-Gaq	Santa Cruz	Cat# sc-393; RRID:AB_631536
Rabbit polyclonal anti-Ga11	Santa Cruz	Cat# sc-394; RRID:AB_2111195
Rabbit polyclonal anti-PLCβ	Santa Cruz	Cat# sc-9050; RRID:AB_2165496
Rabbit polyclonal anti-TRPM8	Alomone labs	Cat# ACC-049; RRID:AB_2040254
Anti-GST HRP conjugated	GE healthcare	Cat# RPN1236; RRID:AB_771429
Mouse monoclonal anti-V5 tag	Thermo Fisher	Cat# R960-25; RRID:AB_2556564
ECL HRP-linked rabbit whole Ab	GE healthcare	Cat# NA934; RRID:AB_772206
ECL HRP-linked mouse whole Ab	GE healthcare	Cat# NA931; RRID:AB_772210
Biological Samples
Mice dorsal root ganglia	This paper	N/A
Chemicals, Peptides, and Recombinant Proteins
DMEM media	Thermo Fisher	Cat# 11564446
Protease inhibitors	Roche Diagnostics	Cat# 11836170001
FBS	Thermo Fisher	Cat# 11550356
TurboFect reagent	Thermo Fisher	Cat# 15325016
Mouse Laminin	Becton Dickinson	Cat# 354232
NGF	Promega	Cat# G5141
Poly-L-lysine	Sigma-Aldrich	Cat# P9155
Cytosine β-D-arabinofuranoside	Sigma-Aldrich	Cat# C1768
Collagenase type IV	Worthington	Cat# LS004186
Bradykinin	Tocris Bioscience	Car# 3004
Rapamycin	Tocris Bioscience	Cat# 1292
Quick-change mutagenesis kit	Agilent Technologies	Cat# 200521
PBMC	Focus Biomolecules	Cat# 10-1413
Glutathione-agarose	Sigma-Aldrich	Cat# G4510
Experimental Models: Cell Lines
HEK293T	ATCC	Cat# CRL11268
Gαq−/− MEF cells	Provided by Prof. Stefan Offermanns	Zywietz et al., 2001
Experimental Models: Organisms/Strains
Gαq knockout mice	Provided by Prof. Stefan Offermanns	Offermanns et al., 1997b
Recombinant DNA
Rat TRPM8-V5-His	Zhang et al., 2012	N/A
G protein alpha q	cDNA resource center	Cat# GNA0Q00000
Bradykinin receptor B2R	cDNA resource center	Cat# BDKB20TN00
Histamine receptor H1R	cDNA resource center	Cat# HRH010TN00
6xHis-tag PLCβ1	Provided by Prof. Elliott M. Ross (UTSMC)	N/A
mRFB-FKBP-5-ptase	Provided by Dr. Gerry Hammond (University of Pittsburgh)	Varnai et al., 2006
PM-FRB-CFP	Provided by Dr. Gerry Hammond	Varnai et al., 2006
Tubby-R332H-cYFP	Provided by Dr. Gerry Hammond	Quinn et al., 2008
GST-pcDNA3	Hasan et al., 2017	N/A
Software and Algorithms
Sigmaplot	Systat Software Inc.	https://systatsoftware.com/products/sigmaplot/
pClamp11	Molecular Device	https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite
Discovery studio 4.5	BIOVIA	https://www.3dsbiovia.com/products/collaborative-science/biovia-discovery-studio/
ImageJ	NIH	https://imagej.nih.gov/ij/
Other
Axopatch 200B amplifier	Molecular Devices	https://www.moleculardevices.com/products/axon-patch-clamp-system/amplifiers/axon-instruments-patch-clamp-amplifiers#gref
Confocal microscope	Leica	https://www.leica-microsystems.com/products/confocal-microscopes/