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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Nat Commun. 2013;4:2384. doi: 10.1038/ncomms3384

Figure 2 |. Metabolomics analysis identifies the alteration of bile-acid composition by tempol.

Figure 2 |

(a) Scatter plot of PLS-DA of fecal ions from vehicle and tempol treatment in HFD-fed mice. Each point represents an individual mouse fecal ion. The labelled ions were identified as β-MCA, DCA, CDCA and γ-MCA, which were decreased by tempol treatment. The p(corr)[1] P-values represent the interclass difference and p[1] P-values represent the relative abundance of the ions. All data were obtained in negative mode (ESI -). (b) Scatter plot of PLS-DA of intestinal ions from vehicle and tempol treatment on a HFD-fed mice. Each point represents an individual mouse intestinal ion. The labelled ions are identified to be T-β-MCA, TCA, TUDCA and TCDCA, which were increased by tempol treatment. The p(corr)[1] values represent the interclass difference and p[1] values represent the relative abundance of the ions. All data are obtained in negative mode (ESI —). (c) Total level of bile acid in feces of the vehicle and tempol group on a HFD for 12 weeks. n =5 mice per group. (d) Total level of bile acid in the intestine of vehicle and tempol group on a HFD for 17 weeks. n = 5 mice per group. (e) Bile-acid composition in fecesof the vehicle and tempol group on a HFD for 12 weeks. n = 5 mice per group. (f) Bile-acid composition in the intestine of vehicle and tempol group on a HFD for 17 weeks. n = 5 mice per group. The total bile acids were quantified by bile-acid kit and bile-acid composition was determined using UPLC-ESI-QTOFMS. All data are presented as mean±s.d., ANOVA followed by two-tailed Student’s t-test. *P<0.05, **P<0.01 compared with vehicle-treated mice.