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. 2019 Jun 4;16:524–534. doi: 10.1016/j.isci.2019.05.045

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Nudt2^fl/fl/CD11c⁺ DCs' Potential in Influencing OT-I CD8⁺ T Cells Antigen Cross-Priming

(A) The percentage of OT-I CD8⁺ T cells that have proliferated following co-culture for 3 days either with Nudt2^fl/fl/CD11c⁺ BMDCs or Nudt2^+/+ CD11c⁺ BMDCs (1:10) incubated with SIINFEKL peptide, OVA and CpG ODN. The harvested cells were analyzed by flow cytometry to quantify CD8⁺ CellTrace Violet⁺ population for proliferation.

(B) Graph represents the percentage of OT-I CD8⁺ T cells proliferated when cultured with Nudt2^+/+ or Nudt2^fl/fl/CD11c⁺ DCs in response to different antigenic preparations.

(C) IL-12 p40 production by DCs during the above co-culture conditions.

(D) mRNA levels for TAP1, TAP2, and TAPASIN in Nudt2^+/+ and Nudt2^fl/fl/CD11c-cre in splenic DCs measured by RT-PCR. Results (mean ± SEM) represent three independent experiments and the significant difference of test in comparision to control. *p < 0.05 (Student's t test or Mann-Whitney test for multiple comparison).