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List of primers used in qRT-PCR.
| Gene | Primers | Annealing temperature(°C) | Product length (bp) |
|---|---|---|---|
| U6 | F:5'GCTTCGGCAGCACATATACTAAAAT3' R:5'CGCTTCACGAATTTGCGTGTCAT3' |
60 | 89 |
| miR-4454 | GSP:5'GGCACGATCCGAGTCACG3' R:5'GTGCGTGTCGTGGAGTCG3' |
60 | 62 |
| miR-451a | GSP:5'GGGGGAAACCGTTACCATTAC3' R:5'GTGCGTGTCGTGGAGTCG3' |
60 | 65 |
| miR-125b-5p | GSP:5'GCTCCCTGAGACCCTAAC3' R:5'GTGCGTGTCGTGGAGTCG3' |
60 | 62 |
