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. 2019 Mar 29;33(5):e22886. doi: 10.1002/jcla.22886

Table 1.

Primer sequences used for real‐time RT‐PCR

Name Sequence (5′‐3′) Expected length
F‐TLR1 TTCACAGTGTCTGGTACACGCAT 101
R‐TLR1 ACCGTGTCTGTTAAGAGATTATTGGA  
F‐TLR2 GCCTCTCCAAGGAAGAATCC 144
R‐TLR2 TCCTGTTGTTGGACAGGTCA  
F‐TLR3 ACAACTTAGCACGGCTCTGGA 124
R‐TLR3 ACCTCAACTGGGATCTCGTCA  
F‐TLR4 AATCTAGAGCACTTGGACCTTTCC 116
R‐TLR4 GGGTTCAGGGACAGGTCTAAAGA  
F‐TLR5 CCATAGATTTTTCCTCCAACCAAATA 141
R‐TLR5 TCATACATTTTCCCCAGTCCACT  
F‐TLR6 CATCCTATTGTGAGTTTCAGGCAT 121
R‐TLR6 GCTTCATAGCACTCAATCCCAAG  
F‐TLR7 GGAGGTATTCCCACGAACACC 141
R‐TLR7 TGACCCCAGTGGAATAGGTACAC  
F‐TLR8 AAACTTGACCCAACTTCGATACCTAA 101
R‐TLR8 GATCCAGCACCTTCAGATGAGG  
F‐TLR9 GGACCTCTGGTACTGCTTCCA 151
R‐TLR9 AAGCTCGTTGTACACCCAGTCT  
F‐TLR10 AAGAAAGGTTCCCGCAGACTT 131
R‐TLR10 TGTTATGGCATAGAATCAAAACTCTCA  
F‐RIG‐I CCATGTAAGACTTGCCTGCTT 74
R‐RIG‐I AAGAGGCTTAATAGATTCACAGTTCC  
F‐NEAT1 CTTCCTCCCTTTAACTTATCCATTCAC 116
R‐NEAT1 CTCTTCCTCCACCATTACCAACAATAC  
F‐GAPDH GCACCGTCAAGGCTGAGAAC 267
F‐GAPDH TGGTGAAGACGCCAGTGGA