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. 2019 Jun 13;2019:8472712. doi: 10.1155/2019/8472712

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Copyright © 2019 Joo Hyun Jee et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of growing mouse gland-derived stomach organoids in Matrigel and collagen-based matrix culture system. (a) Growth pattern and morphology of mouse gland–derived stomach organoids in both Matrigel and collagen-based matrix during Passages 0–4. Bar scale is 50 μm. (b) Quantitative RT-PCR was performed to identify specific markers of stomach organoids at Passage 4 cultured in both matrices (left). Gastric intrinsic factor (gif) was used as a marker of parietal cells, pepsinogen (Pgc) as a marker of chief cells, somatostatin (Stt) as a marker of enteroendocrine cells, ghrelin (Ghrl) as a marker of ghrelin cells, mucin 6 (Muc6) as a marker of gland mucous cells, and mucin 5AC (Muc5AC) for a marker of gastric pit mucous cells. Gene expression shown as gel electroporation (right). ^∗∗p < 0.01, ^∗∗∗p < 0.001. (c) The immunocytochemistry staining of Ki67, mucin 5AC was examined in Matrigel and Collagen matrix. Scale bar: 100 μm.