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. 2019 Jun 13;2019:8472712. doi: 10.1155/2019/8472712

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Copyright © 2019 Joo Hyun Jee et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transplantation of collagen matrix–cultured colon organoids into recipient mouse colonic epithelial. (a) C57BL/6 the recipient tissues were analyzed 7 days after transplantation. EGFP+ engraftments were present in both culture matrices (Matrigel and collagen). (b) Sections of the engrafted tissue 1 week posttransplantation show the presence of EGFP+ crypt cells, Ki67, villin, and chromogranin A differentiated cells in the crypts of donor origin, with Hoechst 33342 (nucleus) staining. Scale bar: 100 μm. (c) Colonic lesion visual analysis of recipient mouse colon. Red box: engraftment site.