Table 1.
Media component.
| Reagent name | Company | Cat. No. | Mouse colon | Mouse small intestine | Mouse stomach | Human colon | Final concentration |
|---|---|---|---|---|---|---|---|
| Advanced DMEM/F12 | Gibco | 12634-028 | O | O | O | O | 1 X |
| Penicillin/streptomycin | Welgene Inc. | LS202-02 | O | O | O | O | 100 U/ml, μg/ml |
| B-27 supplement | Gibco | 17504-044 | O | O | O | O | 1 X |
| N-2 supplement | Gibco | 17502-048 | O | O | O | O | 1 X |
| HEPES buffer solution | Gibco | 15630-080 | O | O | O | O | 10 mM |
| GlutaMAX-I | Gibco | 35050-061 | O | O | O | O | 2 mM |
| N-Acetylcysteine | Sigma-Aldrich | A7250 | O | O | O | O | 1.25 mM |
| Murine recombinant EGF | PeproTech | AF-315-09 | O | O | O | O | 50 ng/ml |
| Murine recombinant Noggin | PeproTech | 250-38 | O | O | O | O | 100 ng/ml |
| R-Spondin-1 conditioned medium | Home-made | O | O | O | O | 10% | |
| Wnt3A conditioned medium | Home-made | O | O | O | 50% | ||
| FGF10, 38-208aa human | ATGen | ATGP1387 | O | 200 ng/ml | |||
| A83-01 | Tocris Bioscience | 2939 | O | O | O | O | 500 nM |
| Nicotinamide | Sigma-Aldrich | N0636 | O | 10 mM | |||
| [Leu15]-Gastrin 1 human | Sigma-Aldrich | G9145 | O | O | 10 nM | ||
| Y-27632 | STEMCELL Technologies | 72304 | O | O | O | O | 10 μM |
| CHIR99021 | Sigma-Aldrich | SML1046 | O | O | O | O | 2.5 μM |
| Thiazovivin | Sigma-Aldrich | SML1045 | O | 2.5 μM | |||
| SB202190 | Sigma-Aldrich | S7067 | O | 10 μM | |||