Figure 6.

FOSL2 Is a Direct Target of miR-143

(A) Bioinformatics analysis revealed the predicted binding sites between FOSL2 and miR-143. (B) Luciferase reporter assay demonstrated miR-143 mimics significantly decreased the luciferase activity of FOSL2-WT in ovarian cancer cells. (C) The real-time PCR analysis was performed to determine the expression levels of FOSL2 in normal ovarian tissues and ovarian cancer tissues from cisplatin-resistant and cisplatin-sensitive patients. (D) Relative expression of FOSL2 in a panel of ovarian cancer cell lines. (E) Overexpression of miR-143 significantly decreased the protein expression of FOSL2 in SKOV-3-DDP cells. All tests were performed at least three times. Data were expressed as mean ± SD. **p < 0.01.