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The susceptibility of ED and FLC against transporter-deletion mutants by spot assay. Cells were adjusted to 1 × 107 cells/ml, 5-fold serially diluted, and spotted onto YPD medium containing 128 μg/ml ED or 1% DMSO (vehicle control). The plates were incubated at 30°C for 48 h. less colony means more sensitive to drug.
