TABLE 1.
Primers and probes for real-time PCR for C. auris detection and sample processing control
| Primer name | Sequence (5′ to 3′)a | Target |
|---|---|---|
| C.auris | For: CGTGATGTCTTCTCACCAATCT | Partial sequence of ITS1 and 28S rRNA gene and complete sequence of 5.8S rRNA gene and ITS2 |
| Rev: TACCTGATTTGAGGCGACAAC | ||
| Pb:6-FAM/TTTGTGAAT/ZEN/GCAACGCCACCGC/IABkFQ | ||
| SPC | For: GGATCTAGCCGTGTGCCCGCT | Not disclosed by BD Max |
| Rev: GGCATGGAGGTTGTCCCATTTGTG | ||
| Pb:ATTO647N TGATGCCTCTTCACATTGCTCCACCTTTCCTBHQ3 |
a
For, forward; Rev, reverse; Pb, probe; SPC, sample processing control; ITS, internal transcribed spacer.