. Author manuscript; available in PMC: 2020 Feb 11.

Published in final edited form as: Dev Cell. 2018 Dec 27;48(3):406–419.e5. doi: 10.1016/j.devcel.2018.11.047

Table 1.

Phenotypic analysis of flies with mutations on modified histone H3 and H4 residues

Mutants	Viability^a	Fertility^b		DNA-damage sensitivity^c		Gene-silencing defects^d

		♂	♀	UV	X-Ray
H3T3A	++	++	sterile	+	+++	+
H3T11A	+	+	sterile	nt	nt	-
H3R17A	++	+	sterile	nt	nt	-
H3K18A	+++	++	++	++	++	-
H3K23A	++	++	+	+	+	+++
H3K56A	++	++	sterile	+++	+++	+++
H3K64A	+++	++	++	+	+	-
H3T107A	++	++	+	+	+++	-
H3K115A	+++	+++	++	+	+++	-
H3K122A	+++	++	++	+	+++	-
H4T1A	++	++	++	+	+++	++
H4R3A	+++	++	+	+	++	-
H4K5A	+++	++	+++	+	+	-
H4K8A	++	+++	+++	++	++	-
H4K16A	++^*	++	+	+	+++	-
H4K20A	+	nt	nt	nt	nt	-
H4R23A	++	+++	+	+	+++	+++
H4K31A	+++	++	++	+	++	+
H4Y51A	++	++	+++	+	++	-
H4K77A	+++	+++	++	++	++	-
H4R92A	+++	++	+	+	++	+



Lethal stage	Mutants

Embryo	H3K4A, H3K14A, H3R26A, H3K27A, H3Y41A, H3T80A, H4Y88A, H4K91A

Larva	H3K9A, H3T45A, H3K37A

Pupa	H3R2A, H3T6A, H3R8A, H3S10A, H3S28A, H3K36A, H3K79A, H4K12A

Viability was represented by the rescue ratios of mutants, which was calculated as the following: ratio = (number of homozygous adult progenies)/(one-third of the number of total adult progenies) × 100. +++ >80%; ++ 30%–80%; + ≤30%.

Fertility was represented by the number of progeny of mutants when crossing with a wild-type counterpart. +++ >80; ++ 30–80; + ≤30; nt not tested.

DNA damage sensitivity was represented by the eclosion ratio, which was calculated as the following: ratio = [E (eclosed pharate number in UV or X-ray group)]/[C (eclosed pharate number in control group)] × 100. Third instar larvae were treated with 100 J/cm² of UV or 30 Gy of X-ray irradiation. +++ <50%; ++ 50–75%; + ≥75%; nt not tested.

Gene silencing was measured by RT-PCR with primers specific to copia retrotransposon. – no changes compared with wild type. + Low expression; ++ Medium expression; +++ Strong expression.

Limited male (<1/1000 viable).