Table 1.

Primer sequences and additional information of the 12 genetic markers

Name	Chr	Repeat motif	SW	Primer sequences (5′–3′)	Allele range
SSchr13b	13	(TTTC)n	522	CTCCAACTCACCTCCAAC AAACACAGGTAAAGAAAGGCCAAA GTTTCTTGAACCCTGGCAACCTTGAA	494–561
SSchr15	15	(TTTC)n	567	AAACCTCTCTCCACACCCAAA GACACATGGATGCTGTTACCTAAA GTTTCTTGATATGAGTA GAGGACAGGAAAGAA	483–524
SSchr1	1	(TCTA)n	531	AACTCCACCACTCCCACAA AACTGAGCCGTTTACAAACCAA GTTTCTTATGGGAATT CCGTTCAGGAAA	353–509
SSchr11	11	(GAAA)n	639	CTCCAACTCACCTCCAACAAA CAAATCTGCACCCAAGTGAA GTTTCTTCTGAAGCA GCATCTGTCTCAA	339–369
SSchr9	9	(GAAA)n	690	AAACCTCTCTCCACACCCAAA ACCACAGATTCCTTCTGCAA GTTTCTTCTGGGATTGC CTCCTTCAA	336–463
SSchr2	2	(TTTC)n	576	CTCACCTCCCACTCCACAAAAT CACTGCTCATTCCGCAAA GTTTCTTATGACATTCC TGTAGAAGGCTGAA	328–389
SSchr14	14	(TTTC)n	612	AACTCCACCACTCCCACAAA ATCCTTCTGCATTTTTCTATCAA GTTTCTTGTCAAAAGTACAT CCCCCTTCCTATAA	332–368
SSchr3	3	(TAGA)n	759	CTCCAACTCACCTCCAACAAA GGGTAGCCCCACCAAAGAA GTTTCTTATTCTGGGA TTAGTGATGCAA	163–227
SSchr8	8	(TAAA)n	500	AAACCTCTCTCCACACCCAA ATGCCTACTACCCCCTTCCAA GTTTCTTCTTGGCTCTT AGGAGGCATAA	187–212
SSchr13a	13	(TAGA)n	558	CTCACCTCCCACTCCACAAAA AATTCTTGGGACTGAAACCAA GTTTCTTCCTCCTTAAT GGGGCTTCTAA	179–203
SSchr5	5	(TAGA)n	504	AACTCCACCACTCCCACAAAG ACCTGGCATCCAAAATCAA GTTTCTTAACCATCAAAAC CCCCTAAA	154–184
SS.Amel	X, Y	N/A	N/A	AACTCCACCACTCCCACAAAG CAGGATCGGTCTGTTTTTC GTTTCTTATGCAAGCCCT CCGAGAA	261, 264

Primer sequences for the 12 genetic markers shown along with their chromosome location (Chr), repeat motif, Smith-Waterman sequence alignment score (SW), and allele range in base pairs. For each marker, the top sequence is the forward primer and bottom sequence is the reverse primer. Section of the forward primers in bold font represents the tag sequence complementary to a universal primer in Table 2. The pigtail sequence GTTTCTT at the 5′ end of the reverse primer is shown in bold font.